2-NAPHTHYL PHOSPHATE CALCIUM SALT - Risk and Safety
WGK Germany
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2-NAPHTHYL PHOSPHATE CALCIUM SALT - Introduction
2-naphthyl phosphate, calcium salt hydrate is an organic phosphate compound. Its chemical formula is C10H6O6P · Ca · xH2O. Where x represents the number of water molecules in the crystal, it can be 0, 1 or 2.
This compound is a white crystalline solid at room temperature and can be dissolved in water. It has a molecular weight of 372.21g/mol. It is stable and can be stored for a long time under dry conditions.
2-napthyl phosphate, calcium salt hydrate is commonly used as a phosphatase substrate in the laboratory. It can be enzymatically hydrolyzed by certain phosphatases to produce phosphate ions and 2-naphthol. This makes it useful as a detection reagent for phosphatase activity in biochemical research and clinical analysis.
The method for preparing 2-naphthyl phosphate, calcium salt hydrate is obtained by the reaction of 2-naphthol and dimethyl calcium phosphate. First, 2-naphthol was suspended in chloroform, and a small amount of anhydrous diethyl ether and dimethylphosphoric acid were added. The reaction mixture was then stirred and cooled to below 0°C. Finally, crystals of 2-naphthyl phosphate, calcium salt hydrate were precipitated by the dropwise addition of ether. The product obtained can be filtered and dried to give the pure compound.
When using and storing 2-napthyl phosphate, sodium salt hydrate, you need to pay attention to some safety matters. It should be operated in a well-ventilated area, avoiding inhalation or contact with skin and eyes. Wear appropriate protective gloves and glasses when using. In addition, such compounds should be kept away from ignition and oxidizing agents and avoid contact with strong acids and bases.